GK097 ScienCell 人類平滑肌細(xì)胞生物學(xué)qPCR檢測試劑盒等地,Human Smooth Muscle Cell Biology qPCR Array Kit
ScienCell 研究實(shí)驗(yàn)室(ScienCell)是一家生物技術(shù)公司合規意識,其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展自動化方案。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞集成,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子互動講,細(xì)胞來源的RNA,cDNA和蛋白質(zhì)像一棵樹。ScienCell的科學(xué)家們以改善生活質(zhì)量過程中,實(shí)現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略能運用。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥達到,公司成立于1999年。
北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商不可缺少,為客戶提供行業分類,正規(guī)進(jìn)口,貨期穩(wěn)定提高鍛煉,折扣好的服務(wù)標(biāo)準(zhǔn)發展邏輯。
GK097 ScienCell人類平滑肌細(xì)胞生物學(xué)qPCR檢測試劑盒,Human Smooth Muscle Cell Biology qPCR Array Kit有所提升,產(chǎn)品簡介:
產(chǎn)品品牌: ScienCell
產(chǎn)品貨號:GK097
產(chǎn)品名稱:GK097 ScienCell人類平滑肌細(xì)胞生物學(xué)qPCR檢測試劑盒聽得進,Human Smooth Muscle Cell Biology qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
北京裕恒豐科技有限公司是ScienCell中國區(qū)總代理,提供各種基因表達(dá)分析相關(guān)品先進水平,ScienCell細(xì)胞培養(yǎng)基便利性、轉(zhuǎn)染試劑全面展示、細(xì)胞檢測試劑盒等細(xì)胞生物學(xué)產(chǎn)品。
GK097 ScienCell人類平滑肌細(xì)胞生物學(xué)qPCR檢測試劑盒深刻認識,Human Smooth Muscle Cell Biology qPCR Array Kit核心技術, 產(chǎn)品說明:
ScienCell 的 GeneQuery™ 人平滑肌細(xì)胞生物學(xué) qPCR 陣列試劑盒 (GQH-SMC) 旨在促進(jìn)參與人類平滑肌細(xì)胞 (SMC) 生物學(xué)的 88 個關(guān)鍵基因的基因表達(dá)譜分析,例如血管 SMC 收縮主動性、SMC 表型切換和細(xì)胞外基質(zhì) (ECM) 合成創造性。與選定的SMC相關(guān)疾病有關(guān)的基因也包含在陣列中。下面顯示了如何根據(jù)其功能對基因進(jìn)行分組的簡要示例:
SMC 標(biāo)記: ACTA2體系, CDH5保障性, CALD1, HEXIM1責任製, HRH2十分落實, MLNR, TAGLN規則製定, DES製造業, CSPG4, SMTN關規定, MKL1/2
血管 SMC 收縮: ANO1發展基礎, ADRA1, NPR1/2建強保護, GNAs同期, MYLKs, ADORA2s使命責任, EDNRA效果, MYH11, MYLs
SMC 表型切換: MYH11情況較常見, MEF2B可持續, SRF, ELK1體製, MYOCD構建, GATA6, MKL1/2服務延伸, RHOA共創輝煌, TGFB1, NOTCH1/3調解製度, HEY2
SMC 異質(zhì)性: ACTA2精準調控, TAGLN, CNN2應用的因素之一, MYH11解決, OGN預期, AQP1, PLN幅度, MGP結構, SPP1, KDR貢獻, SMTN規模最大, VCL, DES
ECM合成:LAMA5統籌,膠原蛋白最深厚的底氣,ELN,OGN振奮起來,MGP品質,SPP1,FGA/B / G
與以下因素有關(guān)的基因
全身性高血壓:PTGIS深入各系統、AGT解決問題、ADD1、AGTR1作用、GNB3相互配合、NOS3
賁門失弛緩癥: NOS1, AAAS著力增加, GMPPA關鍵技術, GUCY1A3, HLA-DQA1/B1
支氣管痙攣/哮喘:HNMT深入、IL13表現、CCL11、ADRB2深刻變革、ALOX5、SCGB3A2
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒和諧共生,每個孔包含一個引物組質生產力,可以特異性識別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計(jì)的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+技術交流,不含DMSO);(ii)除非另有說明先進的解決方案,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證創造更多。
ScienCell's GeneQuery™ Human Smooth Muscle Cell Biology qPCR Array Kit (GQH-SMC) is designed to facilitate gene expression profiling of 88 key genes involved in human smooth muscle cell (SMC) biology, such as vascular SMC contraction, SMC phenotypic switching, and the extracellular matrix (ECM) synthesis. Genes implicated in selected SMC related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:
· SMC markers: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2
· Vascular SMC contraction: ANO1, ADRA1s, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs
· SMC phenotypic switching: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2
· SMC heterogeneity: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES
· ECM synthesis: LAMA5, collagens, ELN, OGN, MGP, SPP1, FGA/B/G
· Genes implicated in
o systemic hypertension: PTGIS, AGT, ADD1, AGTR1, GNB3, NOS3
o achalasia: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1
o bronchospasm/asthma: HNMT, IL13, CCL11, ADRB2, ALOX5, SCGB3A2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查詢™人平滑肌細(xì)胞生物學(xué)qPCR陣列板布局*(8 個粗體和斜體控件)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | AAAS | ADRB2 | AVPR1B | COL3A1 | ELN | GNA13 | HRH2 | MLNR | MYOCD | NPR2 | SCGB3A2 | β-actin |
B | ACTA2 | AEBP1 | CALCRL | COL4A1 | FGA | GNB3 | IL13 | MYH11 | NKX3-1 | OGN | SMAD3 | GAPDH |
C | ADD1 | AGT | CALD1 | CSPG4 | FGB | GUCY1A3 | KDR | MYL6 | NOS1 | PLN | SMTN | LDHA |
D | ADORA2A | AGTR1 | CCL11 | CYP4A11 | FGG | HEXIM1 | LAMA5 | MYL6B | NOS3 | PTGIR | SPP1 | NONO |
E | ADORA2B | ALOX5 | CDH5 | CYP4A22 | GATA6 | HEY2 | MEF2B | MYL9 | NOTCH1 | PTGIS | SRF | PPIH |
F | ADRA1A | ANO1 | CNN2 | DES | GMPPA | HLA-DQA1 | MGP | MYLK | NOTCH3 | RBP1 | TAGLN | GDC |
G | ADRA1B | AQP1 | COL1A1 | EDNRA | GNA11 | HLA-DQB1 | MKL1 | MYLK2 | NOX4 | RBPJ | TGFB1 | PPC |
H | ADRA1D | AVPR1A | COL1A2 | ELK1 | GNA12 | HNMT | MKL2 | MYLK3 | NPR1 | RHOA | VCL | NTC |
提示:基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新
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